Anticorps Polyclonal de lapin anti-SCML1

SCML1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15093-1-PBS

Synonymes

Sex comb on midleg-like protein 1, SCML 1, Gm47, Gm1792

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Galerie de données de validation

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  WB analysis using 15093-1-AP (same clone as 15093-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 15093-1-AP (SCML1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15093-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15093-1-AP (same clone as 15093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15093-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15093-1-AP (same clone as 15093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15093-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 15093-1-AP (same clone as 15093-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SCML1 antibody (15093-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 15093-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 15093-1-AP (same clone as 15093-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug SCML1 Polyclonal antibody (15093-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 15093-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15093-1-PBS cible SCML1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCML1 Protéine recombinante Ag5107
• Nom complet: sex comb on midleg-like 1 (Drosophila)
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC026159
• Symbole du gène: SCML1
• Identification du gène (NCBI): 6322
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.

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