Anticorps Recombinant de lapin anti-SCN4B

SCN4B Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230297B11

N° de cat : 83027-4-PBS

Synonymes

230297B11, Sodium channel subunit beta 4, Sodium channel subunit beta-4

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Galerie de données de validation

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  WB analysis using 83027-4-RR (same clone as 83027-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83027-4-RR (SCN4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83027-4-PBS in a different storage buffer formulation.

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  IHC staining of rat dorsal root ganglion using 83027-4-RR (same clone as 83027-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 83027-4-RR (SCN4B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83027-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83027-4-RR (same clone as 83027-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human SCN4B (83027-4-RR, Clone:230297B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83027-4-PBS in a different storage buffer formulation.

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Informations sur le produit

83027-4-PBS cible SCN4B dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SCN4B Protéine recombinante Ag34123
• Nom complet: sodium channel, voltage-gated, type IV, beta
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: NM_174934.3
• Symbole du gène: SCN4B
• Identification du gène (NCBI): 6330
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming alpha subunit and two auxiliary beta subunits (PMID: 11486343). SCN4B (sodium channel subunit beta-4) is one of several sodium channel beta subunits, which modulate channel gating kinetics. SCN4B has an N-terminal signal peptide, a large extracellular domain predicted to form an Ig-like fold, a single transmembrane alpha helix, and a short cytoplasmic C terminus. Defects in SCN4B gene are a cause of long QT syndrome type 10 (LQT10).

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