Anticorps Monoclonal anti-SCN5A

SCN5A Monoclonal Antibody for Indirect ELISA, IHC, WB

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

Indirect ELISA, IHC, WB

Conjugaison

Non conjugué

CloneNo.

1C2B3

N° de cat : 68273-1-PBS

Synonymes

Nav1.5, HH1, CMPD2, CMD1E, CDCD2

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Galerie de données de validation

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  WB analysis of rabbit heart using 68273-1-Ig (same clone as 68273-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68273-1-Ig (SCN5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 68273-1-Ig (same clone as 68273-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 68273-1-Ig (SCN5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68273-1-Ig (same clone as 68273-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68273-1-Ig (SCN5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68273-1-Ig (same clone as 68273-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN5A (68273-1-Ig, Clone:1C2B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68273-1-PBS cible SCN5A dans les applications de Indirect ELISA, IHC, WB et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SCN5A Protéine recombinante Ag19275
• Nom complet: sodium channel, voltage-gated, type V, alpha subunit
• Masse moléculaire calculée: 2016 aa, 227 kDa
• Poids moléculaire observé: 227 kDa
• Numéro d’acquisition GenBank: BC140813
• Symbole du gène: SCN5A
• Identification du gène (NCBI): 6331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).

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