Anticorps Polyclonal de lapin anti-SCO1

SCO1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 12614-1-PBS

Synonymes

SCOD1

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Galerie de données de validation

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  WB analysis using 12614-1-AP (same clone as 12614-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 12614-1-AP (same clone as 12614-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12614-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 12614-1-AP (same clone as 12614-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 12614-1-AP (same clone as 12614-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 12614-1-AP (same clone as 12614-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12614-1-AP (SCO1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12614-1-PBS cible SCO1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCO1 Protéine recombinante Ag3269
• Nom complet: SCO cytochrome oxidase deficient homolog 1 (yeast)
• Masse moléculaire calculée: 301 aa, 34 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC015504
• Symbole du gène: SCO1
• Identification du gène (NCBI): 6341
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human SCO1 and SCO2 are metallochaperones that are essential for the assembly of the catalytic core of cytochrome c oxidase (COX). SCO1 appears to be involved in the folding and assembly of cytochrome oxidase subunit II, and possibly of other proteins of this subcompartment. It is a 33 kDa protein which displays limited homology to members of the DnaJ family(PMID:14731768). It is also named as SCOD1 and belongs to the SCO1/2 family. It can exsit as a homodimer(PMID:16735468). Defects in SCO1 are a cause of mitochondrial complex IV deficiency (MT-C4D).

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