Anticorps Polyclonal de lapin anti-OXCT1

• Phare
• Validé par KD/KO

OXCT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(56)

Avis (1)

N° de cat : 12175-1-AP

Synonymes

Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, SCOT, OXCT, EC:2.8.3.5, 3-oxoacid CoA-transferase 1

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Galerie de données de validation

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  WB analysis of HeLa using 12175-1-AP

  WB result of OXCT1 antibody (12175-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells.

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  WB analysis using 12175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12175-1-AP (OXCT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12175-1-AP

  IP result of anti-OXCT1 (IP:12175-1-AP, 3ug; Detection:12175-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12175-1-AP (OXCT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12175-1-AP (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (12175-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 12175-1-AP

  Immunofluorescent analysis of MCF-7 cells, using OXCT1 antibody 12175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 12175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (12175-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cardiaque de souris, tissu cérébral de souris, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 50 publications below
• IHC: See 3 publications below
• IF: See 9 publications below

Informations sur le produit

12175-1-AP cible OXCT1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OXCT1 Protéine recombinante Ag2818
• Nom complet: 3-oxoacid CoA transferase 1
• Masse moléculaire calculée: 520 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC009001
• Symbole du gène: OXCT1
• Identification du gène (NCBI): 5019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

Publications

Species	Application	Title
mouse	WB	Nat Med; PMID: 25686106; Authors - Yun-Hee Youm
mouse	WB	Cell Metab; PMID: 32275862; Authors - Rami Al Batran ; KD Validated
mouse	WB,IF	Cell Metab; Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.; Authors - Patrycja Puchalska ; KO Validated
human		Mol Cell; OXCT1 succinylation and activation by SUCLA2 promotes ketolysis and liver tumor growth; Authors - Dong Guo
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
mouse	WB	Sci Adv; Ketogenesis supports hepatic polyunsaturated fatty acid homeostasis via fatty acid elongation; Authors - Eric D Queathem

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kerry (Verified Customer) (09-26-2019)

Too much background. We tried all the dilutions you recommended in your literature and then more outside of both ends of that range.

• Applications: Immunohistochemistry,
• Cell Tissue Type: mouse

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