Anticorps Recombinant de lapin anti-SCP2/SCPx

SCP2/SCPx Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250813C8

N° de cat : 86196-3-RR

Synonymes

SCP2, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16, EC:2.3.1.176

---

Galerie de données de validation

• 

  WB analysis using 86196-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86196-3-RR (SCPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of k562 using 86196-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed k562 using SCP2/SCPx antibody (86196-3-RR, Clone: 250813C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 86196-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86196-3-RR against Human SCP2/SCPx were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules HepG2, cellules MG U-87, cellules NIH/3T3, tissu cérébral de rat
• Résultats positifs en IF/ICC: k562,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86196-3-RR cible SCP2/SCPx dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SCP2/SCPx Protéine recombinante Ag5895
• Nom complet: sterol carrier protein 2
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 46 kDa, 58 kDa
• Numéro d’acquisition GenBank: BC067108
• Symbole du gène: SCP2
• Identification du gène (NCBI): 6342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. It recognizes the intact 58 kDa SCPx protein as well as 44-46 kDa thiolase (PMID: 35996156).

{{ptg:RelatedPrimaryAntibodies}}