Anticorps Recombinant de lapin anti-SCPEP1

SCPEP1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240886B6

N° de cat : 83786-4-PBS

Synonymes

RISC, Retinoid-inducible serine carboxypeptidase, MSTP034, HSCP1, EC:3.4.16.-

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Galerie de données de validation

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  WB analysis of U-251 using 83786-4-RR (same clone as 83786-4-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 83786-4-RR (SCPEP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83786-4-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 83786-4-RR (same clone as 83786-4-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 83786-4-RR (SCPEP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83786-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83786-4-RR (same clone as 83786-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83786-4-RR (SCPEP1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83786-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83786-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83786-4-RR against Human SCPEP1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83786-4-PBS cible SCPEP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SCPEP1 Protéine recombinante Ag31381
• Nom complet: serine carboxypeptidase 1
• Masse moléculaire calculée: 452 aa, 51 kDa
• Poids moléculaire observé: 33 kDa, 45~51 kDa
• Numéro d’acquisition GenBank: BC072405
• Symbole du gène: SCPEP1
• Identification du gène (NCBI): 59342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SCPEP1 encodes retinoid-inducible serine carboxypeptidase (PMID:12975309). SCPEP1 and Cathepsin A play roles in regulation of vasoconstriction via inactivation of endothelin-1 (PubMed:24586188). The protein is widely expressed during mouse embryogenesis by immunohistochemical (PubMed:16461364). Scpep1 is proteolytically cleaved to a mature enzyme which is smaller than 51 kDa (PMID:16461364).

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