Anticorps Recombinant de lapin anti-SCPX

SCPX Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250813B3

N° de cat : 86196-1-RR

Synonymes

SCP2, SCP2/SCPx, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16

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Galerie de données de validation

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  WB analysis using 86196-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 86196-1-RR

  rat liver tissue were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 86196-1-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 86196-1-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 86196-1-RR

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 86196-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 86196-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCPX antibody (86196-1-RR, Clone: 250813B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86196-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86196-1-RR against Human SCPX were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules HepG2, cellules K-562, cellules MG U-87, cellules NIH/3T3, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86196-1-RR cible SCPX dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SCPX Protéine recombinante Ag5895
• Nom complet: sterol carrier protein 2
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 46 kDa, 58 kDa
• Numéro d’acquisition GenBank: BC067108
• Symbole du gène: SCP2
• Identification du gène (NCBI): 6342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCPX protein is a kind of protein related to cholesterol absorption and transport, belonging to SCP-2 gene family. SCPX protein contains an N-terminal thiolase domain and a C-terminal SCP-2 domain. In cells, SCPX protein will be cut into 44-46 kDa thiolase and 13-15 kDa SCP-2 protein. SCPX-2 is a nonspecific lipid transporter, which is involved in the transport and metabolism of intracellular lipids. It is distributed in peroxisome, cytoplasm and nucleus, and may play a role as a transcription factor in nucleus.

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