Anticorps Recombinant de lapin anti-CD138/Syndecan-1

CD138/Syndecan-1 Recombinant Antibody for IHC, FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, FC

Conjugaison

Non conjugué

CloneNo.

240422C4

N° de cat : 98051-1-PBS

Synonymes

CD138, 240422C4, syndecan, SYND1, SDC1

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Galerie de données de validation

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  IHC staining of human tonsillitis using 98051-1-RR (same clone as 98051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98051-1-RR (CD138/Syndecan-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98051-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 98051-1-RR (same clone as 98051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98051-1-RR (CD138/Syndecan-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98051-1-PBS in a different storage buffer formulation.

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  FC experiment of U266 using 98051-1-PBS

  1x10^6 U266 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody, PBS Only (98051-1-PBS, Clone: 240422C4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of U266 using 98051-1-RR (same clone as 98051-1-PBS)

  1x10^6 U266 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody (98051-1-RR, Clone: 240422C4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed. This data was developed using the same antibody clone with 98051-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98051-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98051-1-RR against Human CD138/Syndecan-1 were performed. The affinity constant is 20.8 nM.

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Applications testées

• Résultats positifs en cytométrie: U266 cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98051-1-PBS cible CD138/Syndecan-1 dans les applications de IHC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: syndecan 1
• Masse moléculaire calculée: 32.5 kDa
• Numéro d’acquisition GenBank: BC008765
• Symbole du gène: CD138
• Identification du gène (NCBI): 6382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

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