Anticorps Polyclonal de lapin anti-Syndecan-3

Syndecan-3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (2)

N° de cat : 10886-1-AP

Synonymes

KIAA0468, N syndecan, SDC3, SDCN, SYND3, syndecan 3, Syndecan3, Syndecan-3

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Galerie de données de validation

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  WB analysis using 10886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10886-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 10886-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10886-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10886-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10886-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10886-1-AP

  IP Result of anti-Syndecan-3 (IP:10886-1-AP, 4ug; Detection:10886-1-AP 1:1000) with mouse lung tissue lysate 4000ug.

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  IP experiment of A549 using 10886-1-AP

  IP Result of anti-Syndecan-3 (IP:10886-1-AP, 4ug; Detection:10886-1-AP 1:600) with A549 cells lysate 2800ug.

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  IHC staining of human colon cancer using 10886-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10886-1-AP (Syndecan-3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10886-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10886-1-AP (Syndecan-3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 10886-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-12 cells using 10886-1-AP (Syndecan-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 10886-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SDC3 antibody 10886-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Jurkat, cellules L02, cellules MCF-7, cellules SH-SY5Y, tissu pulmonaire de rat, tissu pulmonaire de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu pulmonaire de souris, cellules A549
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-12, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

10886-1-AP cible Syndecan-3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Syndecan-3 Protéine recombinante Ag1317
• Nom complet: syndecan 3
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC013974
• Symbole du gène: SDC3
• Identification du gène (NCBI): 9672
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.

Publications

Species	Application	Title
human	IHC	Am J Pathol; Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets.; Authors - van Oosterwijk Jolieke G JG
human	IHC	Am J Pathol; No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas.; Authors - Reijnders Christianne M A CM
human	WB	J Cell Sci; Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.; Authors - Yu-Ja Huang
human	IHC	BMC Cancer; Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma.; Authors - Wu Zheng-Sheng ZS
human	IHC	Medicine (Baltimore); Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3; Authors - Guozhi Yang
mouse	IF	Int J Mol Sci; GDNF Increases Inhibitory Synaptic Drive on Principal Neurons in the Hippocampus via Activation of the Ret Pathway; Authors - Apostolos Mikroulis

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:2000 in 1X TBST incubated overnight at 4 degree C. Syndecan-3 appeared at 65 kDa.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WESTERN BLOT (1:2000), IMMUNOFLUORESCENCE (1:100)
• Cell Tissue Type: Mouse liver tissue

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with tris-HCl antigen retrieval ph=6. Co-localisation with microglia based on known markers (not shown).

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina (Microglia) at 3dpf

Syndecan-3 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Microglia) at 3dpf (Cat no:10886-1-AP)