Anticorps Polyclonal de lapin anti-Syntenin 2

• Phare
• Validé par KD/KO

Syntenin 2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 10407-1-AP

Synonymes

Syntenin-2, Syndecan-binding protein 2, SITAC18, SITAC, Similar to TACIP18

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Galerie de données de validation

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  WB analysis of mouse brain using 10407-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10407-1-AP (Syntenin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10407-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10407-1-AP (Syntenin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10407-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10407-1-AP (Syntenin 2 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10407-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10407-1-AP (Syntenin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10407-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10407-1-AP (Syntenin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 10407-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 10407-1-AP (Syntenin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10407-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10407-1-AP (Syntenin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10407-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10407-1-AP (Syntenin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10407-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10407-1-AP (Syntenin 2 antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de souris, tissu hépatique humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de l'estomac humain, tissu de côlon de rat, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10407-1-AP cible Syntenin 2 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Syntenin 2 Protéine recombinante Ag0668
• Nom complet: syndecan binding protein (syntenin) 2
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: BC002727
• Symbole du gène: Syntenin 2
• Identification du gène (NCBI): 27111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syndecan-binding protein 2 (SDCBP2, Syntenin 2) is a protein containing two PDZ domains. PDZ domains facilitate protein-protein interactions by binding to the cytoplasmic C-terminus of transmembrane proteins. SDCBP2 binds with high affinity to PIP2 and is targeted to the plasma membrane, nucleoli and nuclear speckles via its PDZ domains (PMID: 15961997). SDCBP2 exists in two alternatively spliced variants, 2α and 2β, which differ in the lengths of their N-terminal regions. (PMID: 15276154)

Publications

Species	Application	Title
human	WB,IHC	J Virol; Human papillomavirus type 8 E6 oncoprotein inhibits transcription of the PDZ protein syntenin-2.; Authors - Lazi? Daliborka D
human,mouse	WB,IHC,CoIP	Cell Signal; Targeting SDCBP2 in acute myeloid leukemia; Authors - Yan Du ; KD Validated
human	WB, IHC, IF	Front Microbiol; HPV8-E6 Interferes with Syntenin-2 Expression through Deregulation of Differentiation, Methylation and Phosphatidylinositide-Kinase Dependent Mechanisms.; Authors - Benjamin Marx ; KD Validated
human,mouse	WB	Front Neurosci; MiR-409-5p as a Regulator of Neurite Growth Is Down Regulated in APP/PS1 Murine Model of Alzheimer's Disease.; Authors - Jing Guo ; KD Validated
mouse	IF	Gut Pathog; Virulence factors and mechanisms of paediatric pneumonia caused by Enterococcus faecalis; Authors - Zhiying Tian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver tissue lysates were subjected to SDS PAGE followed by western blot with this antibody at dilution of 1:2000 incubated at 4 degree overnight. Syntenin 2 appeared at 36 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver tissue

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