Anticorps Polyclonal de lapin anti-SDHA

• Phare
• Validé par KD/KO

SDHA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

86

Avis (1)

N° de cat : 14865-1-AP

Synonymes

FP, SDH2, SDHA, SDHF

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Galerie de données de validation

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  WB analysis using 14865-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14865-1-AP (SDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14865-1-AP

  IP result of anti-SDHA(IP:14865-1-AP, 4ug; Detection:14865-1-AP 1:20000) with HEK-293 cells lysate 960 ug.

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  IHC staining of human liver cancer using 14865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14865-1-AP (SDHA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14865-1-AP (SDHA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14865-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SDHA antibody (14865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 80 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

14865-1-AP cible SDHA dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SDHA Protéine recombinante Ag6677
• Nom complet: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 62-73 kDa
• Numéro d’acquisition GenBank: BC001380
• Symbole du gène: SDHA
• Identification du gène (NCBI): 6389
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Publications

Species	Application	Title
human	WB	Science; Fumarate is a terminal electron acceptor in the mammalian electron transport chain.; Authors - Jessica B Spinelli ; KO Validated
mouse	WB	Cell Metab; The requirement for pyruvate dehydrogenase in leukemogenesis depends on cell lineage.; Authors - Sojeong Jun
human	WB	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	Mol Cell; Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery; Authors - Jasmin Adriana Schafer
mouse	WB	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jessica (Verified Customer) (08-19-2019)

This antibody worked great for western blots on whole cell lysates and purified mitochondria!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Osteosarcoma cell line

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