Anticorps Monoclonal anti-SDHA

SDHA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5E10G12

N° de cat : 66588-1-PBS

Synonymes

5E10G12, EC:1.3.5.1, FP, MC2DN1, PGL5

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Galerie de données de validation

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  WB analysis using 66588-1-Ig (same clone as 66588-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66588-1-Ig (SDHA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SDHA antibody (66588-1-Ig, Clone: 5E10G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50790-1

  Cytometric bead array standard curve of MP50790-1, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-2-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

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  Cytometric bead array standard curve of MP50790-2

  Cytometric bead array standard curve of MP50790-2, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-3-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

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  Cytometric bead array standard curve of MP50790-3

  Cytometric bead array standard curve of MP50790-3, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-4-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

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Informations sur le produit

66588-1-PBS cible SDHA dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SDHA Protéine recombinante Ag7023
• Nom complet: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 62-73 kDa
• Numéro d’acquisition GenBank: BC001380
• Symbole du gène: SDHA
• Identification du gène (NCBI): 6389
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

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