Anticorps Monoclonal anti-SDHB

• Phare
• Validé par KD/KO

SDHB Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1A2A5

N° de cat : 67600-1-PBS

Synonymes

1A2A5, EC:1.3.5.1, Iron-sulfur subunit of complex II, PGL 4, PGL4

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Galerie de données de validation

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  WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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  WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67600-1-Ig (same clone as 67600-1-PBS)

  WB result of SDHB antibody (67600-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SDHB transfected HepG2 cells. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67600-1-Ig (same clone as 67600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67600-1-Ig (SDHB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67600-1-Ig (same clone as 67600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67600-1-Ig (SDHB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67600-1-Ig (same clone as 67600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67600-1-Ig (SDHB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67600-1-Ig (same clone as 67600-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SDHB antibody (67600-1-Ig, Clone: 1A2A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67600-1-PBS cible SDHB dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SDHB Protéine recombinante Ag29868
• Nom complet: succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC007840
• Symbole du gène: SDHB
• Identification du gène (NCBI): 6390
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).

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