Anticorps Recombinant de lapin anti-SEC13

SEC13 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242775C3

N° de cat : 85110-4-PBS

Synonymes

242775C3, D3S1231E, GATOR2 complex protein SEC13, Protein SEC13 homolog, SEC13A

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Galerie de données de validation

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  WB analysis using 85110-4-RR (same clone as 85110-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85110-4-RR (SEC13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85110-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85110-4-RR (same clone as 85110-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85110-4-RR (SEC13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85110-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85110-4-RR (same clone as 85110-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85110-4-RR (SEC13 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85110-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85110-4-RR (same clone as 85110-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC13 antibody (85110-4-RR, Clone: 242775C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85110-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85110-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85110-4-RR against Human SEC13 were performed. The affinity constant is 0.114 nM.

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Informations sur le produit

85110-4-PBS cible SEC13 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SEC13 Protéine recombinante Ag7610
• Nom complet: SEC13 homolog (S. cerevisiae)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC002634
• Symbole du gène: SEC13
• Identification du gène (NCBI): 6396
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)

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