Anticorps Polyclonal de lapin anti-SEC16A

• Phare
• Validé par KD/KO

SEC16A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 20025-1-AP

Synonymes

SEC16L, SEC16 homolog A, SEC16, RP11 413M3.10, p250

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Galerie de données de validation

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  WB analysis of MCF-7 using 20025-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20025-1-AP (SEC16A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20025-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20025-1-AP (SEC16A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20025-1-AP

  IP result of anti-SEC16A (IP:20025-1-AP, 4ug; Detection:20025-1-AP 1:2000) with HeLa cells lysate 840 ug.

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  IHC staining of mouse pancreas using 20025-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 20025-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 20025-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 20025-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SEC16A antibody (20025-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 20025-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SEC16A (20025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

20025-1-AP cible SEC16A dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SEC16 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 234 kDa
• Poids moléculaire observé: 250-300 kDa
• Numéro d’acquisition GenBank: NM_014866
• Symbole du gène: SEC16A
• Identification du gène (NCBI): 9919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SEC16A, also named as KIAA0310, SEC16 and SEC16L, is required for secretory cargo trafic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. SEC16A is required for normal transitional endoplasmic reticulum (tER) organization.

Publications

Species	Application	Title
human	WB,IF	Nat Chem Biol; Functional dissection of the retrograde Shiga toxin trafficking inhibitor Retro-2.; Authors - Alison Forrester ; KD Validated
rat	WB	Cell Death Differ; C9orf72 controls hepatic lipid metabolism by regulating SREBP1 transport; Authors - Yachen Wu
mouse	IF	Mol Neurodegener; Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency; Authors - Panpan Wang

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