Anticorps Recombinant de lapin anti-SEC22B

SEC22B Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris, Hamster

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242659B3

N° de cat : 85037-5-PBS

Synonymes

242659B3, ER-Golgi SNARE of 24 kDa, ERS24, ERS-24, SEC22 vesicle-trafficking protein homolog B

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Galerie de données de validation

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  WB analysis using 85037-5-RR (same clone as 85037-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85037-5-RR (SEC22B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 85037-5-RR (same clone as 85037-5-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SEC22B Recombinant antibody (85037-5-RR, Clone:242659B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85037-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85037-5-RR against Human SEC22B were performed. The affinity constant is 2.07 nM.

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Informations sur le produit

85037-5-PBS cible SEC22B dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris, Hamster

• Réactivité: Humain, rat, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SEC22B Protéine recombinante Ag12981
• Nom complet: SEC22 vesicle trafficking protein homolog B (S. cerevisiae)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC001364
• Symbole du gène: SEC22B
• Identification du gène (NCBI): 9554
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) proteins are critical components of the membrane fusion machinery (PMID: 20163564). SEC22B is a SNARE involved in endoplasmic reticulum (ER)/Golgi membrane trafficking. SEC22B localizes to the ER-Golgi intermediate compartment (ERGIC) and has been identified as a regulator of antigen crosspresentation (PMID: 22153078).

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