Anticorps Monoclonal anti-SEC24D

SEC24D Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G5G9

N° de cat : 67409-1-PBS

Synonymes

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

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Galerie de données de validation

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  WB analysis of Jurkat using 67409-1-Ig (same clone as 67409-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67409-1-Ig (same clone as 67409-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67409-1-Ig (same clone as 67409-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67409-1-Ig (same clone as 67409-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC24D antibody (67409-1-Ig, Clone: 3G5G9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67409-1-PBS cible SEC24D dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SEC24D Protéine recombinante Ag4657
• Nom complet: SEC24 family, member D (S. cerevisiae)
• Masse moléculaire calculée: 1033 aa, 113 kDa
• Poids moléculaire observé: 113 kDa
• Numéro d’acquisition GenBank: BC035761
• Symbole du gène: SEC24D
• Identification du gène (NCBI): 9871
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

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