Anticorps Recombinant de lapin anti-SEC31A

SEC31A Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242531E2

N° de cat : 84942-4-PBS

Synonymes

242531E2, ABP125, ABP130, KIAA0905, SEC31 like protein 1

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Galerie de données de validation

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  WB analysis using 84942-4-RR (same clone as 84942-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84942-4-RR (SEC31A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84942-4-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 84942-4-RR (same clone as 84942-4-PBS)

  IP result of anti-SEC31A (IP:84942-4-RR, 4ug; Detection:84942-4-RR 1:4000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 84942-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84942-4-RR (same clone as 84942-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SEC31A antibody (84942-4-RR, Clone: 242531E2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84942-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84942-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84942-4-RR against Human SEC31A were performed. The affinity constant is 12.4 nM.

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Informations sur le produit

84942-4-PBS cible SEC31A dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SEC31A Protéine recombinante Ag12314
• Nom complet: SEC31 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 1220 aa, 133 kDa
• Poids moléculaire observé: 118-140 kDa
• Numéro d’acquisition GenBank: BC084583
• Symbole du gène: SEC31A
• Identification du gène (NCBI): 22872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). The sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologs of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.

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