Anticorps Polyclonal de lapin anti-SEC5/EXOC2

• Phare
• Validé par KD/KO

SEC5/EXOC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12751-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse brain using 12751-1-AP (same clone as 12751-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12751-1-AP (same clone as 12751-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

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  WB analysis of human ileum using 12751-1-AP (same clone as 12751-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12751-1-AP (same clone as 12751-1-PBS)

  IP result of anti-SEC5/EXOC2 (IP:12751-1-AP, 5ug; Detection:12751-1-AP 1:500) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12751-1-AP (same clone as 12751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12751-1-AP (same clone as 12751-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12751-1-PBS cible SEC5/EXOC2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC5/EXOC2 Protéine recombinante Ag3147
• Nom complet: exocyst complex component 2
• Masse moléculaire calculée: 924 aa, 104 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC016918
• Symbole du gène: SEC5
• Identification du gène (NCBI): 55770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.

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