Anticorps Polyclonal de lapin anti-SEC6

• Phare
• Validé par KD/KO

SEC6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14703-1-PBS

Synonymes

EXOC3, Exocyst complex component 3, Exocyst complex component Sec6, SEC6L1

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Galerie de données de validation

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  WB analysis of human brain using 14703-1-AP (same clone as 14703-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:86 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  WB analysis using 14703-1-AP (same clone as 14703-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14703-1-AP (same clone as 14703-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14703-1-AP (same clone as 14703-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 14703-1-AP (same clone as 14703-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14703-1-AP (SEC6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14703-1-AP (same clone as 14703-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 14703-1-AP (SEC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14703-1-PBS cible SEC6 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC6 Protéine recombinante Ag6383
• Nom complet: exocyst complex component 3
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC064569
• Symbole du gène: SEC6
• Identification du gène (NCBI): 11336
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.

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