Anticorps Polyclonal de lapin anti-SEC61B

SEC61B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (2)

N° de cat : 15087-1-AP

Synonymes

Sec61 beta subunit, SEC61B

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Galerie de données de validation

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  WB analysis using 15087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15087-1-AP (SEC61B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15087-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15087-1-AP (SEC61B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15087-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15087-1-AP (SEC61B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15087-1-AP

  IP Result of anti-SEC61B (IP:15087-1-AP, 4ug; Detection:15087-1-AP 1:500) with HepG2 cells lysate 1800ug.

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  IHC staining of human liver using 15087-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15087-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 15087-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 15087-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 15087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (15087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 15087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (15087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SEC61B antibody (15087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 15087-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15087-1-AP (SEC61B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules L02, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa, tissu pancréatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

15087-1-AP cible SEC61B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC61B Protéine recombinante Ag7169
• Nom complet: Sec61 beta subunit
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 10-15 kDa
• Numéro d’acquisition GenBank: BC001734
• Symbole du gène: SEC61B
• Identification du gène (NCBI): 10952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).

Publications

Species	Application	Title
human	WB	Science; QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis.; Authors - Kwontae You
mouse	WB	Nat Neurosci; Faulty autolysosome acidification in Alzheimer's disease mouse models induces autophagic build-up of Aβ in neurons, yielding senile plaques.; Authors - Ju-Hyun Lee
human	WB	Sci Adv; CReP mediates selective translation initiation at the endoplasmic reticulum.; Authors - Jonathan P Kastan
human	IF	J Cell Biol; Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway.; Authors - Satoshi Ninagawa
human	IF	Genes Dev; Identification of a localized nonsense-mediated decay pathway at the endoplasmic reticulum.; Authors - Dasa Longman
human,mouse	WB	Mol Ther; Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation.; Authors - Xinjie Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (09-22-2020)

SEC61B Antibody have correct localization on ER.

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse heart

FH

WEI (Verified Customer) (06-29-2020)

Strong band for heart tissues, but not so sharp band.

• Applications: Western Blot,
• Cell Tissue Type: Mouse heart

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