Anticorps Polyclonal de lapin anti-SEC61B

SEC61B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 51020-2-AP

Synonymes

Protein transport protein Sec61 subunit beta

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Galerie de données de validation

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  WB analysis using 51020-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51020-2-AP (SEC61B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 51020-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 51020-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 51020-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 51020-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pancreas using 51020-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse pancreas using 51020-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 51020-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 51020-2-AP (SEC61B antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules 3T3-L1, cellules BxPC-3, cellules C2C12, cellules C6, cellules HEK-293T, cellules HeLa, cellules HepG2, cellules RAW 264.7
• Résultats positifs en IHC: tissu pancréatique de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu pancréatique de souris,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

51020-2-AP cible SEC61B dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Sec61 beta subunit
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 10-15 kDa
• Numéro d’acquisition GenBank: BC001734
• Symbole du gène: SEC61B
• Identification du gène (NCBI): 10952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).

Publications

Species	Application	Title
mouse	IF	J Exp Med; Host ER-parasitophorous vacuole interaction provides a route of entry for antigen cross-presentation in Toxoplasma gondii-infected dendritic cells.; Authors - Goldszmid Romina S RS
human	WB	EMBO Rep; UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation; Authors - Brittany A Ahlstedt
mouse	WB	Sci Rep; Neuroprotection mediated by cystatin C-loaded extracellular vesicles.; Authors - Rocío Pérez-González
mouse	WB	Methods Mol Biol; Assessing Rab5 Activation in Health and Disease; Authors - Anna Pensalfini
mouse	WB	Nat Commun; The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition; Authors - Rakesh Ganji

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