Anticorps Polyclonal de lapin anti-SEC61B

SEC61B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 51020-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 51020-2-AP (same clone as 51020-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51020-2-AP (SEC61B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 51020-2-AP (same clone as 51020-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 51020-2-AP (same clone as 51020-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 51020-2-AP (same clone as 51020-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 51020-2-AP (SEC61B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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Informations sur le produit

51020-2-PBS cible SEC61B dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Sec61 beta subunit
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 10-15 kDa
• Numéro d’acquisition GenBank: BC001734
• Symbole du gène: SEC61B
• Identification du gène (NCBI): 10952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).

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