Anticorps Polyclonal de lapin anti-SEC61B-Specific

SEC61B-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 14846-1-AP

Synonymes

Sec61 beta subunit, SEC61B, SEC61B-Specific

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Galerie de données de validation

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  WB analysis using 14846-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14846-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14846-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 14846-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 14846-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14846-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 14846-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 14846-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14846-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse pancreas using 14846-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse pancreas using 14846-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14846-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IHC: tissu hépatique de souris, tissu de cancer du col de l'utérus humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu pancréatique de souris, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

14846-1-AP cible SEC61B-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Sec61 beta subunit
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 10-15 kDa
• Numéro d’acquisition GenBank: NM_006808
• Symbole du gène: SEC61B
• Identification du gène (NCBI): 10952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).

Publications

Species	Application	Title
human	WB	PLoS Pathog; Legionella hijacks the host Golgi-to-ER retrograde pathway for the association of Legionella-containing vacuole with the ER.; Authors - Mio Kawabata

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