Anticorps Polyclonal de lapin anti-SEC63

SEC63 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28665-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 28665-1-AP (same clone as 28665-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28665-1-AP (SEC63 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 28665-1-AP (same clone as 28665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 28665-1-AP (same clone as 28665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 28665-1-AP (same clone as 28665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 28665-1-AP (same clone as 28665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 28665-1-AP (same clone as 28665-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (28665-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

28665-1-PBS cible SEC63 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC63 Protéine recombinante Ag30213
• Nom complet: SEC63 homolog (S. cerevisiae)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC047221
• Symbole du gène: SEC63
• Identification du gène (NCBI): 11231
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

{{ptg:RelatedPrimaryAntibodies}}