Anticorps Monoclonal anti-SEC63

SEC63 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1C10E1

N° de cat : 67352-1-PBS

Synonymes

1C10E1, DnaJ homolog subfamily C member 23, DNAJC23, SEC63L, Translocation protein SEC63 homolog

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Galerie de données de validation

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  WB analysis using 67352-1-Ig (same clone as 67352-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67352-1-Ig (SEC63 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67352-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67352-1-Ig (same clone as 67352-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SEC63 antibody (67352-1-Ig, Clone: 1C10E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67352-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67352-1-PBS cible SEC63 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SEC63 Protéine recombinante Ag5783
• Nom complet: SEC63 homolog (S. cerevisiae)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC047221
• Symbole du gène: SEC63
• Identification du gène (NCBI): 11231
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

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