Anticorps Polyclonal de lapin anti-E-selectin/CD62E

E-selectin/CD62E Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(48)

Avis (1)

N° de cat : 20894-1-AP

Synonymes

E-selectin / CD62E, CD62E, E-selectin, CD62 antigen-like family member E, ELAM1

---

Galerie de données de validation

• 

  WB analysis using 20894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20894-1-AP (E-selectin/CD62E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human appendicitis using 20894-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 20894-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 20894-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 20894-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HUVEC,
• Résultats positifs en IHC: tissu d'appendicite humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 39 publications below
• IHC: See 4 publications below
• IF: See 9 publications below

Informations sur le produit

20894-1-AP cible E-selectin/CD62E dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: E-selectin/CD62E Protéine recombinante Ag14944
• Nom complet: selectin E
• Masse moléculaire calculée: 610 aa, 67 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC142711
• Symbole du gène: CD62E
• Identification du gène (NCBI): 6401
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury; Authors - Xun Wu
human	WB	Sci Adv; The support of genetic evidence for cardiovascular risk induced by antineoplastic drugs.; Authors - Hui Cui
human	IF	Adv Healthc Mater; Boosting Tumor Accumulation of Phthalocyanine through Sialylation Engineering for Superior Cancer Phototherapy; Authors - Jian Li
	WB	ACS Appl Mater Interfaces; Inflammation-Driven Nanohitchhiker Enhances Postoperative Immunotherapy by Alleviating Prostaglandin E2-Mediated Immunosuppression; Authors - Yingke Liu
human	IF	Biomater Sci; An E-selectin targeting and MMP-2-responsive dextran-curcumin polymeric prodrug for targeted therapy of acute kidney injury; Authors - Jing-Bo Hu
human	WB	Genomics; MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.; Authors - Yuan Shi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Yannic (Verified Customer) (01-07-2022)

Left and right band refer to markers. Many unspecific bands, blocked with 5% BSA, primary antibody over night

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HMEC1 endothelial cells

E-selectin/CD62E Antibody Western Blot validation (1:1000 dilution) in HMEC1 endothelial cells (Cat no:20894-1-AP)

{{ptg:RelatedPrimaryAntibodies}}