Anticorps Polyclonal de lapin anti-SELK

SELK Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 12561-1-AP

Synonymes

Selenoprotein K, HSPC297, HSPC030

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Galerie de données de validation

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  WB analysis of K-562 using 12561-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12561-1-AP (SELK antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

12561-1-AP cible SELK dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SELK Protéine recombinante Ag3143
• Nom complet: selenoprotein K
• Masse moléculaire calculée: 94 aa, 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC013162
• Symbole du gène: SELK
• Identification du gène (NCBI): 58515
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Selenoprotein K (SelK), a single-pass transmembrane protein that resides in the endoplasmic reticulum (ER) membrane, which has been reported to an emerging role of playing a part in protein palmitoylation. Human SelK is a 94-amino-acid protein with Sec residing at the penultimate position. It has been shown that SelK is expressed predominantly in the heart and skeletal muscle, but other tissues, such as the liver, pancreas, and placenta, also have detectable levels of SelK.(PMID: 36252341)

Publications

Species	Application	Title
human,mouse	WB	Biochem Biophys Res Commun; Knockdown of Trnau1ap inhibits the proliferation and migration of NIH3T3, JEG-3 and Bewo cells via the PI3K/Akt signaling pathway.; Authors - Xiaoyan Hu
mouse	WB	Front Pharmacol; Prominent Effects of Berbamine Hydrochloride on Alzheimer's Disease Model Mice.; Authors - Chang-lan Chen
rat	WB	Cell Mol Life Sci; Selenium-SelK-GPX4 axis protects nucleus pulposus cells against mechanical overloading-induced ferroptosis and attenuates senescence of intervertebral disc; Authors - Chunwang Jia
human	WB	Redox Rep; Selenium participates in the formation of kidney stones by alleviating endoplasmic reticulum stress and apoptosis of renal tubular epithelial cells; Authors - Xiaozhe Su ; KD Validated

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