Anticorps Polyclonal de lapin anti-P-selectin / CD62P

P-selectin / CD62P Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13304-1-AP

Synonymes

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP

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Galerie de données de validation

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  WB analysis of human peripheral blood platelets using 13304-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: plaquettes de sang périphérique humain,
• Résultats positifs en IHC: tissu splénique humain, tissu d'amygdalite humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• FC: See 2 publications below

Informations sur le produit

13304-1-AP cible P-selectin / CD62P dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P-selectin / CD62P Protéine recombinante Ag4124
• Nom complet: selectin P (granule membrane protein 140kDa, antigen CD62)
• Masse moléculaire calculée: 830 aa, 91 kDa
• Numéro d’acquisition GenBank: BC028067
• Symbole du gène: SELP
• Identification du gène (NCBI): 6403
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Publications

Species	Application	Title
mouse	WB	ACS Appl Mater Interfaces; AIEgen-Based Bionic Nanozymes for the Interventional Photodynamic Therapy-Based Treatment of Orthotopic Colon Cancer.; Authors - Yanhong Duo
human	WB	Int J Biol Macromol; Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing; Authors - Hang Dong
human	FC	World J Gastroenterol; Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer; Authors - Jia-Cheng Li
human	IF,FC	Immun Inflamm Dis; Neutrophil extracellular traps induce thrombogenicity in severe carotid stenosis.; Authors - Shihua Zhang
human	WB	Nat Commun; Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions; Authors - Chinnaswamy Tiruppathi

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