Anticorps Monoclonal anti-P-selectin / CD62P

P-selectin / CD62P Monoclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

19

CloneNo.

3G2C7

N° de cat : 60322-1-Ig

Synonymes

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP

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Galerie de données de validation

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  WB analysis using 60322-1-Ig

  Untreated and PNGase F-treated lysates of human platelets were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  WB analysis of human peripheral blood platelets using 60322-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60322-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 60322-1-Ig

  IP Result of anti-P-selectin / CD62P (IP:60322-1-Ig, 5ug; Detection:60322-1-Ig 1:600) with K-562 cells lysate 1760ug.

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Applications testées

• Résultats positifs en WB: plaquettes de sang périphérique humain, cellules Jurkat
• Résultats positifs en IP: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• FC: See 4 publications below

Informations sur le produit

60322-1-Ig cible P-selectin / CD62P dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P-selectin / CD62P Protéine recombinante Ag4124
• Nom complet: selectin P (granule membrane protein 140kDa, antigen CD62)
• Masse moléculaire calculée: 830 aa, 91 kDa
• Poids moléculaire observé: 140 kDa, 105-110 kDa
• Numéro d’acquisition GenBank: BC028067
• Symbole du gène: SELP
• Identification du gène (NCBI): 6403
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Publications

Species	Application	Title
rat	WB	Acta Biomater; Rescuing ischemic stroke by biomimetic nanovesicles through accelerated thrombolysis and sequential ischemia-reperfusion protection.; Authors - Wenyan Yu
human	WB	Front Oncol; An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.; Authors - Hongwei Jin
mouse	WB	Front Oncol; Platelet Membranes Coated Gold Nanocages for Tumor Targeted Drug Delivery and Amplificated Low-Dose Radiotherapy.; Authors - Mingzhu Chen
human	FC	Commun Biol; Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.; Authors - LinXi Cheng
mouse	WB	Front Bioeng Biotechnol; Nano-Platelets as an Oxygen Regulator for Augmenting Starvation Therapy Against Hypoxic Tumor.; Authors - Chunyu Huang
human	WB	Biol Res; CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis.; Authors - Xiaohao Zhang

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