Anticorps Recombinant de lapin anti-SELPLG/CD162

SELPLG/CD162 Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241297G10

N° de cat : 84500-1-PBS

Synonymes

SELPLG, 241297G10, CD162, CLA, P-selectin glycoprotein ligand 1

---

Galerie de données de validation

• 

  IHC staining of human tonsillitis using 84500-1-RR (same clone as 84500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84500-1-RR (SELPLG/CD162 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84500-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84500-1-RR (same clone as 84500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84500-1-RR (SELPLG/CD162 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84500-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 84500-1-RR (same clone as 84500-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SELPLG antibody (84500-1-RR, Clone: 241297G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84500-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84500-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84500-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.221 nM.

---

Informations sur le produit

84500-1-PBS cible SELPLG/CD162 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: selectin P ligand
• Masse moléculaire calculée: 412 aa, 43 kDa
• Numéro d’acquisition GenBank: BC029782
• Symbole du gène: SELPLG
• Identification du gène (NCBI): 6404
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).

{{ptg:RelatedPrimaryAntibodies}}