Anticorps Polyclonal de lapin anti-SEMA3A

SEMA3A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27836-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 27836-1-AP (same clone as 27836-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27836-1-AP (SEMA3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 27836-1-AP (same clone as 27836-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEMA3A antibody (27836-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 27836-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

27836-1-PBS cible SEMA3A dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEMA3A Protéine recombinante Ag27173
• Nom complet: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• Masse moléculaire calculée: 771 aa, 89 kDa
• Poids moléculaire observé: 89 and 65 kDa
• Numéro d’acquisition GenBank: BC111416
• Symbole du gène: SEMA3A
• Identification du gène (NCBI): 10371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).

{{ptg:RelatedPrimaryAntibodies}}