Anticorps Recombinant de lapin anti-SEMA3A

SEMA3A Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240509E3

N° de cat : 83579-5-PBS

Synonymes

SEMA1, Sema III, Hsema III, Hsema I, coll 1

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Galerie de données de validation

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  WB analysis using 83579-5-RR (same clone as 83579-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83579-5-RR (SEMA3A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83579-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83579-5-RR (same clone as 83579-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83579-5-RR (SEMA3A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83579-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 83579-5-RR (same clone as 83579-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83579-5-RR (SEMA3A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83579-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83579-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83579-5-RR against Human SEMA3A were performed. The affinity constant is 0.228 nM.

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Informations sur le produit

83579-5-PBS cible SEMA3A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SEMA3A Protéine recombinante Ag27173
• Nom complet: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• Masse moléculaire calculée: 771 aa, 89 kDa
• Poids moléculaire observé: 89 kDa
• Numéro d’acquisition GenBank: BC111416
• Symbole du gène: SEMA3A
• Identification du gène (NCBI): 10371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).

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