Anticorps Recombinant de lapin anti-SEMA4A

SEMA4A Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250325B12

N° de cat : 98513-2-PBS

Synonymes

Sema B, Semab, Semaphorin-4A, Semaphorin-B, SemB

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98513-2-RR (same clone as 98513-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

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  FC experiment of mouse splenocytes using 98513-2-RR (same clone as 98513-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98513-2-RR (same clone as 98513-2-PBS)

  1x10^6 HEK-293T cells or SEMA4A transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse Sema4a Rabbit RecAb (98513-2-RR, Clone: 250325B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98513-2-RR (same clone as 98513-2-PBS)

  1x10^6 HEK-293T cells (left) or SEMA4A transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98513-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98513-2-RR against Mouse SEMA4A were performed. The affinity constant is below 1 pM.

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Informations sur le produit

98513-2-PBS cible SEMA4A dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SEMA4A Protéine recombinante Eg2680
• Nom complet: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
• Masse moléculaire calculée: 83 kDa
• Numéro d’acquisition GenBank: NM_001163489.1
• Symbole du gène: Sema4a
• Identification du gène (NCBI): 20351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

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