Anticorps Recombinant de lapin anti-SEMA4A

SEMA4A Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250325B12

N° de cat : 98513-2-RR

Synonymes

Sema B, Semab, Semaphorin-4A, Semaphorin-B, SemB

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98513-2-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD3. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98513-2-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98513-2-RR

  1x10^6 HEK-293T cells or SEMA4A transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse Sema4a Rabbit RecAb (98513-2-RR, Clone: 250325B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98513-2-RR

  1x10^6 HEK-293T cells (left) or SEMA4A transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98513-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98513-2-RR against Mouse SEMA4A were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris, Transfected HEK-293T cells

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98513-2-RR cible SEMA4A dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SEMA4A Protéine recombinante Eg2680
• Nom complet: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
• Masse moléculaire calculée: 83 kDa
• Numéro d’acquisition GenBank: NM_001163489.1
• Symbole du gène: Sema4a
• Identification du gène (NCBI): 20351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

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