Anticorps Polyclonal de lapin anti-SEMA4C

SEMA4C Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28402-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 28402-1-AP (same clone as 28402-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 28402-1-AP (same clone as 28402-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  WB analysis using 28402-1-AP (same clone as 28402-1-PBS)

  Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28402-1-PBS cible SEMA4C dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEMA4C Protéine recombinante Ag29143
• Nom complet: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
• Masse moléculaire calculée: 833 aa, 93 kDa
• Poids moléculaire observé: 93 kDa
• Numéro d’acquisition GenBank: BC109104
• Symbole du gène: SEMA4C
• Identification du gène (NCBI): 54910
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SEMA4C is a member of transmembrane semaphorin proteins which regulate axonal guidance in the developing nervous system. Furthermore, studies have demonstrated that sema4C was also implicated in biological events outside the nervous system, such as cell migration, tumor development, and terminal myogenic differentiation (PMID: 36010604). SEMA4C is highly expressed in breast, lung, cervical, and liver cancers. SEMA4C is involved in oncogenic signaling and is a potential therapeutic target for invasive breast cancer, hepatocellular carcinoma, glioma, and osteosarcoma (PMID: 33177246).

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