Anticorps Recombinant de lapin anti-SENP1

SENP1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240478D3

N° de cat : 83594-4-PBS

Synonymes

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3

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Galerie de données de validation

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  WB analysis using 83594-4-RR (same clone as 83594-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83594-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83594-4-RR (same clone as 83594-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83594-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83594-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83594-4-RR against Human SENP1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83594-4-PBS cible SENP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SENP1 Protéine recombinante Ag18028
• Nom complet: SUMO1/sentrin specific peptidase 1
• Masse moléculaire calculée: 644 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC045639
• Symbole du gène: SENP1
• Identification du gène (NCBI): 29843
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

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