Anticorps Polyclonal de lapin anti-SENP3

• Phare
• Validé par KD/KO

SENP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 17659-1-AP

Synonymes

SUMO-1-specific protease 3, SSP3, SMT3IP1, Sentrin-specific protease 3, Sentrin/SUMO-specific protease SENP3

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Galerie de données de validation

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  WB analysis using 17659-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17659-1-AP (SENP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17659-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17659-1-AP (SENP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 17659-1-AP

  IP result of anti-SENP3 (IP:17659-1-AP, 4ug; Detection:17659-1-AP 1:500) with Y79 cells lysate 2000ug.

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  IHC staining of human ovary cancer using 17659-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17659-1-AP (SENP3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17659-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17659-1-AP (SENP3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Y79, tissu embryonnaire de rat
• Résultats positifs en IP: cellules Y79
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

17659-1-AP cible SENP3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SENP3 Protéine recombinante Ag11911
• Nom complet: SUMO1/sentrin/SMT3 specific peptidase 3
• Masse moléculaire calculée: 574 aa, 65 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC080658
• Symbole du gène: SENP3
• Identification du gène (NCBI): 26168
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SENP3(Sentrin-specific protease 3) is also named as SSP3, SUSP3 and belongs to the peptidase C48 family. It acts as an essential factor for ribosome biogenesis. SENP3 might regulate the sumoylation of other substrates through directly binding or mediating association with NPM1 at the nucleolus and other subcellular compartments.

Publications

Species	Application	Title
human	WB	Cell Death Differ; Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4; Authors - Wei Liu
human	WB	Mol Cell Proteomics; Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.; Authors - Fanis Pavlos P
	WB	Int J Oncol; Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9; Authors - Zhenwei Ma
human	WB,IF	FASEB J; SUMO1 SUMOylates and SENP3 deSUMOylates NLRP3 to orchestrate the inflammasome activation.; Authors - Luyao Shao
human	WB	Breast Cancer Res Treat; Targeting PELP1 oncogenic signaling in TNBC with the small molecule inhibitor SMIP34; Authors - Kristin A Altwegg
mouse	WB,IF	iScience; SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury; Authors - Qian Xia ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293 and HeLa cells, and primary rat cortical neurons

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