Anticorps Polyclonal de lapin anti-SENP8

SENP8 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 13479-1-AP

Synonymes

DEN1, Deneddylase 1, HsT17512, NEDD8 specific protease 1, NEDP1, Protease, cysteine 2, PRSC2, SENP8, Sentrin specific protease 8

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Galerie de données de validation

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  IHC staining of human colon cancer using 13479-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13479-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13479-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 13479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 13479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat pancreas using 13479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 13479-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 13479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SENP8 antibody (13479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu rénal de rat, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu hépatique humain, tissu pancréatique de rat, tissu pancréatique de souris, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13479-1-AP cible SENP8 dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SENP8 Protéine recombinante Ag4287
• Nom complet: SUMO/sentrin specific peptidase family member 8
• Masse moléculaire calculée: 212 aa, 24 kDa
• Poids moléculaire observé: 24-28 kDa
• Numéro d’acquisition GenBank: BC031411
• Symbole du gène: SENP8
• Identification du gène (NCBI): 123228
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SENP8, a human NEDD8-specific protease, also known as NEDP1. It is expected to be located in cytoplasm and nucleus, which is highly expressed in kidney and pancreas. The calculated molecular weight of SENP8 is 24 kDa. It is a protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53 (PMID: 15242646). A single-residue difference in the C-terminus of NEDD8 and ubiquitin contributes significantly to the ability of NEDP1 to discriminate between them. In vivo analysis indicates that NEDP1 mutants perturb deNEDDylation of the tumour suppressor p53 (PMID: 12759363).