Anticorps Polyclonal de lapin anti-Septin 6

• Phare
• Validé par KD/KO

Septin 6 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(16)

Avis (1)

N° de cat : 12805-1-AP

Synonymes

KIAA0128, SEP2, SEPT2, SEPT6, septin 6, septin6

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Galerie de données de validation

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  WB analysis of Jurkat using 12805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12805-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12805-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 12805-1-AP

  IP result of anti-Septin 6 (IP:12805-1-AP, 4ug; Detection:12805-1-AP 1:600) with rat brain tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 12805-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (Septin 6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12805-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (SEPT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

12805-1-AP cible Septin 6 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Septin 6 Protéine recombinante Ag3521
• Nom complet: septin 6
• Masse moléculaire calculée: 434 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC036240
• Symbole du gène: SEPT6
• Identification du gène (NCBI): 23157
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.

Publications

Species	Application	Title
human	WB	J Biol Chem; Uncovering principles that control septin-septin interactions.; Authors - Moshe S Kim
human,rat	WB	Lab Invest; Effect of SEPT6 on the biological behavior of hepatic stellate cells and liver fibrosis in rats and its mechanism.; Authors - Yuhui Fan ; KD Validated
mouse	WB	Proteomics; Quantification of protein isoforms in mesenchymal stem cells by reductive dimethylation of lysines in intact proteins.; Authors - She Yi-Min YM
human	IHC	Int J Oncol; SEPT6 drives hepatocellular carcinoma cell proliferation, migration and invasion via the Hippo/YAP signaling pathway.; Authors - Yuhui Fan
human	WB	Mol Biol Cell; Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties.; Authors - Forooz Soroor
human	IF	Mol Hum Reprod; The SEPT12 complex is required for the establishment of a functional sperm head-tail junction.; Authors - Yi-Ru Shen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mohammed (Verified Customer) (08-19-2020)

Did not show strong staining at the sperm annulus. Overall very high background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: (1:200)
• Cell Tissue Type: Testis

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