Anticorps Recombinant de lapin anti-Septin 9

• Phare
• Validé par KD/KO

Septin 9 Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E7

N° de cat : 82844-2-PBS

Synonymes

SEPT9, 2E7, KIAA0991, MLL septin-like fusion protein, MLL septin-like fusion protein MSF-A

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Galerie de données de validation

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  WB analysis using 82844-2-RR (same clone as 82844-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 82844-2-RR (same clone as 82844-2-PBS)

  WB result of SEPT9 antibody (82844-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEPT9 transfected A549 cells. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

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  WB analysis using 82844-2-RR (same clone as 82844-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 82844-2-RR (same clone as 82844-2-PBS)

  IP result of anti-SEPT9 (IP:82844-2-RR, 4ug; Detection:82844-2-RR 1:1000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82844-2-RR (same clone as 82844-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82844-2-RR (same clone as 82844-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82844-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82844-2-RR against Human SEPT9 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82844-2-PBS cible Septin 9 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Septin 9 Protéine recombinante Ag1218
• Nom complet: septin 9
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 65 kDa, 38-40 kDa
• Numéro d’acquisition GenBank: BC021192
• Symbole du gène: SEPT9
• Identification du gène (NCBI): 10801
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

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