Anticorps Monoclonal anti-SERCA2,ATP2A2

SERCA2,ATP2A2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F11A11

N° de cat : 67248-1-PBS

Synonymes

ATP2A2, SERCA2, SERCA2b, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Calcium pump 2

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Galerie de données de validation

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  WB analysis of HEK-293 using 67248-1-Ig (same clone as 67248-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67248-1-Ig (same clone as 67248-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67248-1-Ig (same clone as 67248-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67248-1-Ig (same clone as 67248-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67248-1-Ig (same clone as 67248-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 67248-1-Ig (same clone as 67248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 67248-1-Ig (same clone as 67248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67248-1-Ig (same clone as 67248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 67248-1-Ig (same clone as 67248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67248-1-PBS cible SERCA2,ATP2A2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SERCA2,ATP2A2 Protéine recombinante Ag5052
• Nom complet: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Masse moléculaire calculée: 1042 aa, 115 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC035588
• Symbole du gène: SERCA2
• Identification du gène (NCBI): 488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.

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