Anticorps Monoclonal anti-SERCA3

SERCA3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A2C2

N° de cat : 68287-1-PBS

Synonymes

ATP2A3, Calcium pump 3, SERCA3, SR Ca(2+) ATPase 3

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Galerie de données de validation

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  WB analysis using 68287-1-Ig (same clone as 68287-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68287-1-Ig (ATP2A3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68287-1-PBS in a different storage buffer formulation.

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  WB analysis using 68287-1-Ig (same clone as 68287-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68287-1-Ig (ATP2A3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68287-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 68287-1-Ig (same clone as 68287-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SERCA3 antibody (68287-1-Ig, Clone: 1A2C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68287-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68287-1-PBS cible SERCA3 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SERCA3 Protéine recombinante Ag4527
• Nom complet: ATPase, Ca++ transporting, ubiquitous
• Masse moléculaire calculée: 1043 aa, 114 kDa
• Poids moléculaire observé: 97-110 kDa
• Numéro d’acquisition GenBank: BC035729
• Symbole du gène: SERCA3
• Identification du gène (NCBI): 489
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The SERCA, which pumps cytosolic Ca2+ into the ER, is an imperative maintainer of ER Ca2+ homeostasis. The mammalian SERCA family is comprised of three tissue-specific members, SERCA1-3. SERCA3 is present in endothelial and epithelial cells of various tissues. SERCA3 helps regulate intracellular Ca2+, which is critical for maintaining normal INS secretion and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. Several isoforms of SERCA3 exist due to the alternative splicing.

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