Anticorps Polyclonal de lapin anti-SERP1

SERP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17807-1-PBS

Synonymes

RAMP4, Ribosome-attached membrane protein 4, Stress-associated endoplasmic reticulum protein 1

---

Galerie de données de validation

• 

  WB analysis of mouse brain using 17807-1-AP (same clone as 17807-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 17807-1-AP (same clone as 17807-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 17807-1-AP (same clone as 17807-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 17807-1-AP (same clone as 17807-1-PBS)

  IP result of anti-SERP1 (IP:17807-1-AP, 4ug; Detection:17807-1-AP 1:500) with mouse brain tissue lysate 3160 ug. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 17807-1-AP (same clone as 17807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17807-1-AP (SERP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 17807-1-AP (same clone as 17807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17807-1-AP (SERP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 17807-1-AP (same clone as 17807-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SERP1 antibody (17807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

17807-1-PBS cible SERP1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SERP1 Protéine recombinante Ag12087
• Nom complet: stress-associated endoplasmic reticulum protein 1
• Masse moléculaire calculée: 66 aa, 7 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC108314
• Symbole du gène: SERP1
• Identification du gène (NCBI): 27230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Stress-associated endoplasmic reticulum (ER) protein 1 (SERP1), also known as ribosome- associated membrane protein 4 (RAMP4), is a Sec61-associated polypeptide that is induced by ER stress [PMID:16705175]. SERP1 interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. It controls glycosylation of major histocompatibility complex class II-associated invariant chains by a translocational pausing mechanism, and its overexpression stabilizes newly synthesized membrane proteins under ER stress by associating with the Sec61 complex [PMID:10601334]. It is suggested SERP1 is involved in the biosynthesis/processing of secretory proteins

{{ptg:RelatedPrimaryAntibodies}}