Anticorps Polyclonal de lapin anti-Alpha Antichymotrypsin

Alpha Antichymotrypsin Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 12192-1-AP

Synonymes

A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3

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Galerie de données de validation

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  WB analysis of human plasma using 12192-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12192-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12192-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 12192-1-AP

  IP Result of anti-alpha 1 Antichymotrypsin (IP:12192-1-AP, 4ug; Detection:12192-1-AP 1:500) with COLO 320 cells lysate 2000ug.

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  IHC staining of human tonsillitis using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HepG2 using 12192-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Alpha Antichymotrypsin (12192-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: tissu sanguin humain, cellules COLO 320, tissu de côlon humain
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu hépatique humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

12192-1-AP cible Alpha Antichymotrypsin dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha Antichymotrypsin Protéine recombinante Ag2830
• Nom complet: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
• Masse moléculaire calculée: 448 aa, 48 kDa
• Poids moléculaire observé: 44-70 kDa
• Numéro d’acquisition GenBank: BC010530
• Symbole du gène: SERPINA3
• Identification du gène (NCBI): 12
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.

Publications

Species	Application	Title
		Acta Neuropathol; Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS; Authors - Amr Aly
human	WB	Front Immunol; Screening and identification of tissue-infiltrating immune cells and genes for patients with emphysema phenotype of COPD; Authors - Di Wang
human	WB,IHC	Br J Cancer; Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.; Authors - Yanxia Jin
mouse	IHC	Front Med (Lausanne); An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.; Authors - Songyan Gao
		Proteomics Clin Appl; Serum protein profiling in diffuse large B-cell lymphoma.; Authors - Jacques Riby
human,rat	WB	Food Funct; Sinensetin attenuates IL-1β-induced cartilage damage and ameliorates osteoarthritis by regulating SERPINA3; Authors - Zhendong Liu