Anticorps Monoclonal anti-SERPINB9

SERPINB9 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H9D2

N° de cat : 67950-1-PBS

Synonymes

CAP 3, CAP3, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI 9, PI9, Serpin B9, SERPINB9

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Galerie de données de validation

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  WB analysis of K-562 using 67950-1-Ig (same clone as 67950-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 67950-1-Ig (same clone as 67950-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 67950-1-Ig (same clone as 67950-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 67950-1-Ig (same clone as 67950-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67950-1-Ig (same clone as 67950-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 67950-1-Ig (same clone as 67950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 67950-1-Ig (same clone as 67950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  IF Staining of C6 using 67950-1-Ig (same clone as 67950-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SERPINB9 antibody (67950-1-Ig, Clone: 2H9D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67950-1-PBS cible SERPINB9 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SERPINB9 Protéine recombinante Ag6719
• Nom complet: serpin peptidase inhibitor, clade B (ovalbumin), member 9
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC002538
• Symbole du gène: SERPINB9
• Identification du gène (NCBI): 5272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SERPINB9, also named as PI-9, belongs to the serpin family. SERPINB9 is an intracellular inhibitor of granzyme B (grB) that protects cytotoxic lymphocytes from grB-mediated death. It has a nucleo-cytoplasmic distribution and is produced in CD8+ T cells and natural killer cells (PMID: 28024184). Also, SERPINB9 is expressed by vascular SMCs and endothelial cells to avoid GZB-induced apoptosis (PMID: 28743062). The molecular weight of SERPINB9 is 42 kDa.

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