Anticorps Monoclonal anti-SERPINE2

SERPINE2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E11F12

N° de cat : 66203-1-PBS

Synonymes

GDN, Glia derived nexin, Peptidase inhibitor 7, PI 7, PI7, PN 1, PN1, PN-1, PNI, Protease nexin 1, Protease nexin I, Serpin E2, SERPINE2

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Galerie de données de validation

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  WB analysis using 66203-1-Ig (same clone as 66203-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66203-1-Ig (same clone as 66203-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66203-1-Ig (same clone as 66203-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 66203-1-Ig (same clone as 66203-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66203-1-Ig (same clone as 66203-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66203-1-Ig (same clone as 66203-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66203-1-Ig (same clone as 66203-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66203-1-Ig (same clone as 66203-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66203-1-Ig (same clone as 66203-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SERPINE2 antibody (66203-1-Ig, Clone: 1E11F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66203-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66203-1-PBS cible SERPINE2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SERPINE2 Protéine recombinante Ag1830
• Nom complet: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC015663
• Symbole du gène: SERPINE2
• Identification du gène (NCBI): 5270
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

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