Anticorps Polyclonal de lapin anti-SESN1

SESN1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 21668-1-AP

Synonymes

p53 regulated protein PA26, PA26, RP11 787I22.1, SESN1, SEST1, sestrin 1

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Galerie de données de validation

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  WB analysis using 21668-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 21668-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 21668-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21668-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain, cellules HEK-293, cellules HeLa, cellules K-562, tissu hépatique de souris, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 2 publications below

Informations sur le produit

21668-1-AP cible SESN1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SESN1 Protéine recombinante Ag16462
• Nom complet: sestrin 1
• Masse moléculaire calculée: 551 aa, 64 kDa
• Poids moléculaire observé: 66-68 kDa
• Numéro d’acquisition GenBank: BC112036
• Symbole du gène: SESN1
• Identification du gène (NCBI): 27244
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids).

Publications

Species	Application	Title
mouse	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
mouse	WB	Science; Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.; Authors - Andrew L Cangelosi
		Diabetes; PMID: 25377878; Authors - Rongya Tao
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner; Authors - Sujuan Liu
human	WB,IHC	Osteoarthritis Cartilage; Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.; Authors - T Shen
human	WB	BMC Cancer; Inhibition of mTORC1 through ATF4-induced REDD1 and Sestrin2 expression by Metformin.; Authors - Se-Kyeong Jang