Anticorps Recombinant de lapin anti-SESN3

SESN3 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242189D1

N° de cat : 84823-3-PBS

Synonymes

242189D1, EC:1.11.1.-, SEST3, sestrin 3, Sestrin-3

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Galerie de données de validation

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  WB analysis using 84823-3-RR (same clone as 84823-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84823-3-RR (SESN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84823-3-RR (same clone as 84823-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84823-3-RR (SESN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84823-3-RR (same clone as 84823-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SESN3 antibody (84823-3-RR, Clone: 242189D1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84823-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84823-3-RR against Human SESN3 were performed. The affinity constant is 6.78 nM.

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Informations sur le produit

84823-3-PBS cible SESN3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SESN3 Protéine recombinante Ag17872
• Nom complet: sestrin 3
• Masse moléculaire calculée: 492 aa, 57 kDa
• Poids moléculaire observé: 57-60 kDa
• Numéro d’acquisition GenBank: BC017296
• Symbole du gène: SESN3
• Identification du gène (NCBI): 143686
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT-induced increase in ROS. It might be a promising target in selectively killing cancer cells with high AKT activity levels.

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